Electrochemical ELISA is an enzyme linked immunosorbent assay (ELISA) designed for use in low resource settings and at the point of care. It is fabricated on hydrophobic paper made by silanizing paper with decyl trichlorosilane. The device consists of an embossed microwell and two zones separated by a central crease. The device is folded along the crease, contacting the two zones, and then recording the results.
The handheld ELISA device couples most common electrochemical techniques directly to the "cloud" using audio and vibration. It is compatible with any mobile phone or cellular network. It allows for quantitative sensing and easy transfer of data. The Electrochemical ELISA method has many benefits, including its affordability and ability to perform routine analysis anywhere in the world. In addition to the ability to be used in a variety of contexts, Electrochemical ELISA is versatile and can be used in personal health, environmental monitoring, and clinical analysis.
This method is used to detect protein biomarkers in biological samples. The system uses biomarkers such as glucose, BSA, and HBsAg. This biomarker ELISA allows the researchers to identify specific proteins and biomarkers by measuring the protein level in the blood. This method is widely used to monitor the activity of cancer cells, and it has been used to detect tumor markers. This biomarker is highly sensitive and has a wide range.
The development of new diagnostic technologies is essential for managing the COVID-19 pandemic. For example, a new point of care Electrochemical ELISA platform uses disposable screen-printed electrodes and glycoprotein S1 spike to detect COVID-19 antibodies. The test is validated against 3 FDA-authorized laboratory standard techniques, including EDTA whole blood samples and serum. When compared to the results of 3 different methods, the Point of Care Electrochemical ELISA platform shows superior performance and validated as a rapid and accurate diagnostic tool. To prove the accuracy of the subsequent detection process, scientists always use a plate washer to clean the residues on the plate.
Previous studies performed on DSPP in GCF used ELISA with spectrophotometry. However, they were difficult to quantify. Electrochemical ELISA was specifically designed to identify DSPP in orthodontic patients. This technology is highly sensitive and reliable. This technique has many advantages over spectrophotometric detection. The technology has many applications, from clinical diagnosis to pharmaceutical development. The electrochemical ELISA is ideal for small samples.
Another advantage of Electrochemical ELISA is that it is fast. The total incubation time was only 25 minutes, compared to 90 minutes for the optical immunoassay. Additionally, the sensitivity and specificity of the test make it an excellent choice for rapid screening. The technology has the potential to be portable and disposable. It may contribute to the prevention and control of Ostertagia ostertagi infection in cattle.
A gold-film array-electrode is being developed for Electrochemical ELISA. The method allows for the amperometric quantification of oxidized 3,5,3',5'-tetramethylbenzidine, a widely used chromogenic substrate for horseradish peroxidase. The gold-film electrode for the working electrode is made by sputtering gold on a PEI strip. The reference electrode is produced by spin-coating the sample with Ag/AgCl paste.